Intestinal absorption of vitamin B6. I. Pyridoxol uptake by rat intestinal tissue.

The uptake of 3H-pyridoxol by everted intestinal rings of the rat was studied mainly at concentrations of 0.1-4ƒÊM of the vitamin in the medium. Incubation of the tissue at 37•Ž for 60 min in Krebs-Ringer bicarbonate medium containing glucose and labelled pyridoxol resulted in intracellular accumulation of the labelled compounds. The total intracellular concentration of the compounds after the incubation was always higher than the extracellular concentration, and this accumu lation was especially appreciable at relatively low external concentra tions of the 3H-vitamin. The uptake under the same conditions was